enzyme linked immunosorbent assay elisa plate reader  (Bio-Rad)

Journal: ImmunoHorizons

Article Title: NRF2 agonists 4-octyl-itaconate and dimethyl fumarate reduce human and bovine RSV proliferation and RSV disease in a murine model

doi: 10.1093/immhor/vlaf036

Figure Lengend Snippet: Effects of intranasal 4-OI and DMF administration in the inflammatory response and H 2 O 2 production in RSV infected mice lungs. (A) RNA and cDNA were processed from mice lungs (n = 9) and transcription of inflammatory cytokines was determined through RT-PCR. Expression level was normalized to vehicle-treated, uninfected cells, and beta-actin (mouse) was used as a housekeeping gene. Statistical analyses results are shown in . (B) IL-6 protein levels were quantified in mice lung lysates (n = 8–11) through an indirect ELISA. P values were obtained from a 1-way analysis of variance followed by Dunnett’s multiple comparisons test. For A and B, data show the mean ± SEM 3 independent experiments. (C) H 2 O 2 levels were measured in mice lung lysates (n = 6) using a colorimetric assay. P values were obtained from a Kruskal-Wallis test. Data show the median ± range from 2 dependent experiments. All data points were plotted.

Article Snippet: Finally, the well content was transferred to a flat-bottomed 96-well plate to be read at 570 nm in an enzyme-linked immunosorbent assay (ELISA) microplate reader (Fisher Scientific accuSkan FC Filter-Based Microplate Photometer).

Techniques: Infection, Reverse Transcription Polymerase Chain Reaction, Expressing, Indirect ELISA, Colorimetric Assay